This article focuses on the relation between laccase-secreting ability and growing cycle in the edible fungi. First, laccase activities of fifteen different edible fungi were detected and determined by plate assay and spectrophotometric method using 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) as the substrate. The results showed the laccase-secreting ability in the edible fungi and their growing cycles are closely related. The edible fungi strains with short growing cycles originate from their high levels of secreted laccase activity. However, those strains require long growing cycles due to the low levels of secreted laccase, even no detectable laccase activity. The research provides the first evidence on the corresponding relation between the level of secreted laccase activity and growth cycles of edible fungi. Our study has significantly increased the understanding of the role of laccase in the growth and development of edible fungi.