Abstract
In this study, we identified p53 as a novel TCTP-interacting protein using TCTP as bait. Also, we determined the critical binding sites between TCTP and p53. To elucidate the functional consequence of the interaction, we developed the overexpression and inhibition system of TCTP and p53 expression. Overexpression of TCTP in lung carcinoma cells reversed p53 mediated apoptosis and inhibition of TCTP expression by small interfering RNA increased apoptosis of lung carcinoma cells. Moreover, it was observed that TCTP overexpression promotes degradation of p53. These results clearly indicate that the interaction between TCTP and p53 prevents apoptosis by destabilizing p53. Thus, TCTP acts as a negative regulator of apoptosis in lung cancer.
Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Apoptosis Regulatory Proteins / genetics
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Apoptosis Regulatory Proteins / metabolism*
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Apoptosis*
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Binding Sites / genetics
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Biomarkers, Tumor / genetics
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Biomarkers, Tumor / metabolism*
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Blotting, Western
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Cell Line, Tumor
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Cell Proliferation
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DNA Fragmentation
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Humans
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Immunoprecipitation
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Molecular Sequence Data
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Protein Binding
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Protein Interaction Mapping
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Protein Stability
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RNA Interference
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Transfection
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Tumor Protein, Translationally-Controlled 1
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Tumor Suppressor Protein p53 / genetics
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Tumor Suppressor Protein p53 / metabolism*
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Two-Hybrid System Techniques
Substances
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Apoptosis Regulatory Proteins
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Biomarkers, Tumor
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TPT1 protein, human
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Tumor Protein, Translationally-Controlled 1
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Tumor Suppressor Protein p53
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Green Fluorescent Proteins