Matrix-assisted laser desorption ionization-time of flight mass spectrometry as an alternative to 16S rRNA gene sequencing for identification of difficult-to-identify bacterial strains

A Bizzini; K Jaton; D Romo; J Bille; G Prod'hom; G Greub

doi:10.1128/JCM.01463-10

Matrix-assisted laser desorption ionization-time of flight mass spectrometry as an alternative to 16S rRNA gene sequencing for identification of difficult-to-identify bacterial strains

J Clin Microbiol. 2011 Feb;49(2):693-6. doi: 10.1128/JCM.01463-10. Epub 2010 Nov 24.

Authors

A Bizzini¹, K Jaton, D Romo, J Bille, G Prod'hom, G Greub

Affiliation

¹ Institute of Microbiology, University of Lausanne and University Hospital Center, Rue du Bugnon 46, 1011 Lausanne, Switzerland.

Abstract

Conventional methods are sometimes insufficient to identify human bacterial pathogens, and alternative techniques, often molecular, are required. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identified with a valid score 45.9% of 410 clinical isolates from 207 different difficult-to-identify species having required 16S rRNA gene sequencing. MALDI-TOF MS might represent an alternative to 16S rRNA gene sequencing.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / chemistry*
Bacteria / classification*
Bacteria / isolation & purification
Bacterial Infections / diagnosis*
Bacterial Infections / microbiology*
Bacteriological Techniques / methods*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Humans
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

DNA, Bacterial
DNA, Ribosomal
RNA, Ribosomal, 16S