Abstract
We developed a series of plasmids that allow C-terminal tagging of any gene in its endogenous locus in Giardia intestinalis, with different epitope tags (triple hemagglutinin [3HA] and triple Myc [3Myc]) and selection markers (puromycin, neomycin, and a newly developed marker, blasticidin). Using these vectors, cyclin B and aurora kinase were tagged, expressed, and localized.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Aurora Kinases
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Cyclin B / genetics
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Cyclin B / metabolism
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Gene Knock-In Techniques
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Genes, Protozoan*
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Genetic Engineering
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Genetic Markers
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Genetic Vectors
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Giardia lamblia / genetics*
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Giardia lamblia / metabolism
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism
Substances
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Cyclin B
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Genetic Markers
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Aurora Kinases
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Protein Serine-Threonine Kinases