Abstract
In this study, the effect of aucubin on H(2)O(2)-induced apoptosis was studied by using a rat pheochromocytoma (PC12) cell line. We have analyzed the apoptosis of H(2)O(2)-induced PC12 cells, H(2)O(2)-induced apoptosis appeared to correlate with lower Bcl-2 expression, higher Bax expression and sequential activation of caspase-3 leading to cleavage of poly-ADP-ribose polymerase (PARP). Aucubin not only inhibited lower Bcl-2 expression, high Bax expression, but also modulated caspase-3 activation, PARP cleavage, and eventually protected against H(2)O(2)-induced apoptosis. These results indicated that aucubin can obstruct H(2)O(2)-induced apoptosis by regulating of the expression of Bcl-2 and Bax, as well as suppression of caspases cascade activation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis / drug effects*
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Caspases / metabolism*
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Cell Survival / drug effects
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Chromatin / ultrastructure
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Hydrogen Peroxide / pharmacology*
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Iridoid Glucosides / chemistry
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Iridoid Glucosides / pharmacology*
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Molecular Structure
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Oxidants / pharmacology
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PC12 Cells / drug effects*
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PC12 Cells / physiology
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PC12 Cells / ultrastructure
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Poly(ADP-ribose) Polymerases / metabolism
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Proto-Oncogene Proteins c-bcl-2 / genetics
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Proto-Oncogene Proteins c-bcl-2 / metabolism*
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Rats
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Signal Transduction / drug effects*
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bcl-2-Associated X Protein / metabolism
Substances
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Chromatin
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Iridoid Glucosides
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Oxidants
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Proto-Oncogene Proteins c-bcl-2
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bcl-2-Associated X Protein
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aucubin
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Hydrogen Peroxide
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Poly(ADP-ribose) Polymerases
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Caspases