Abstract
Understanding the mechanisms of augmented bacterial pathogenicity in post-viral infections is the first step in the development of an effective therapy. This study assessed the effect of human coronavirus NL63 (HCoV-NL63) on the adherence of bacterial pathogens associated with respiratory tract illnesses. It was shown that HCoV-NL63 infection resulted in an increased adherence of Streptococcus pneumoniae to virus-infected cell lines and fully differentiated primary human airway epithelium cultures. The enhanced binding of bacteria correlated with an increased expression level of the platelet-activating factor receptor (PAF-R), but detailed evaluation of the bacterium-PAF-R interaction revealed a limited relevance of this process.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Adhesion*
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Cell Line
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Cells, Cultured
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Coronavirus NL63, Human / physiology*
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Epithelial Cells / metabolism
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Epithelial Cells / microbiology*
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Epithelial Cells / virology*
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Gene Expression
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Humans
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Platelet Membrane Glycoproteins / genetics
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Platelet Membrane Glycoproteins / metabolism
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Receptors, G-Protein-Coupled / genetics
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Receptors, G-Protein-Coupled / metabolism
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Respiratory Tract Infections / genetics
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Respiratory Tract Infections / metabolism
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Respiratory Tract Infections / microbiology*
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Respiratory Tract Infections / virology*
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Streptococcus / physiology*
Substances
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Platelet Membrane Glycoproteins
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Receptors, G-Protein-Coupled
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platelet activating factor receptor