Biogenic amines content in Spanish and French natural ciders: application of qPCR for quantitative detection of biogenic amine-producers

Food Microbiol. 2011 May;28(3):554-61. doi: 10.1016/j.fm.2010.11.005. Epub 2010 Nov 19.

Abstract

Biogenic amines (BA) are low molecular weight nitrogenous bases commonly found in fermented foods and beverages and their consumption can induce undesirable reactions. In this work, the BA content in natural cider from Spain and France was determined. Samples from commercially available cider or obtained during the elaboration process were analyzed. A different profile and BA concentration was observed depending on cider origin. qPCR tools developed for the quantitative detection of BA producers from cheese and wine were tested in the cider samples. A good connection between the BA content and the presence of BA-producing microorganisms was observed. Based on these tools, BA-producing bacteria were isolated from the analyzed cider samples, including new potential histamine- and putrescine-producing Lactobacillus paracollinoides strains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Beverages / analysis*
  • Biogenic Amines / analysis*
  • Biogenic Amines / biosynthesis
  • Cheese / microbiology
  • Chromatography, High Pressure Liquid
  • Consumer Product Safety
  • Fermentation
  • Food Analysis / methods
  • Food Microbiology
  • France
  • Humans
  • Lactobacillus / metabolism*
  • Malus
  • Polymerase Chain Reaction*
  • Spain
  • Wine / microbiology

Substances

  • Biogenic Amines