Abstract
HIV-1 p17 contains C- and N-terminal sequences with positively charged residues and a consensus cluster for heparin binding. We have previously demonstrated by affinity chromatography that HIV-1 p17 binds strongly to heparin-agarose at physiological pH and to human activated CD4(+) T cells. In this study we demonstrated that the viral protein binds to heparan sulfate side chains of syndecan-2, syndecan-4, and CD44v3 purified from HeLa cells and that these heparan sulfate proteoglycans (HSPGs) co-localize with HIV-1 p17 on activated human CD4(+) T cells by confocal fluorescence analysis. Moreover, we observed a stimulatory or inhibitory activity when CD4(+) T cells were activated with mitogens together with nanomolar or micromolar concentrations of the matrix protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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CD4-Positive T-Lymphocytes / immunology
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CD4-Positive T-Lymphocytes / metabolism*
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Female
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HIV Antigens / genetics
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HIV Antigens / immunology
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HIV Antigens / metabolism*
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HIV-1 / genetics
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HIV-1 / immunology
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HIV-1 / metabolism*
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HeLa Cells
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Heparitin Sulfate / genetics
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Heparitin Sulfate / immunology
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Heparitin Sulfate / metabolism*
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Humans
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Hyaluronan Receptors / genetics
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Hyaluronan Receptors / immunology
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Hyaluronan Receptors / metabolism*
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Interleukin-2 / biosynthesis*
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Interleukin-2 / genetics
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Interleukin-2 / immunology
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Lymphocyte Activation
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Male
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Protein Binding
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Syndecan-2 / genetics
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Syndecan-2 / immunology
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Syndecan-2 / metabolism*
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Syndecan-4 / genetics
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Syndecan-4 / immunology
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Syndecan-4 / metabolism*
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Tumor Necrosis Factor-alpha / biosynthesis*
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Tumor Necrosis Factor-alpha / genetics
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Tumor Necrosis Factor-alpha / immunology
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gag Gene Products, Human Immunodeficiency Virus / genetics
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gag Gene Products, Human Immunodeficiency Virus / immunology
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gag Gene Products, Human Immunodeficiency Virus / metabolism*
Substances
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CD44V3,8-10
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HIV Antigens
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Hyaluronan Receptors
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IL2 protein, human
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Interleukin-2
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SDC2 protein, human
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SDC4 protein, human
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Syndecan-4
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Tumor Necrosis Factor-alpha
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gag Gene Products, Human Immunodeficiency Virus
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p17 protein, Human Immunodeficiency Virus Type 1
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Syndecan-2
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Heparitin Sulfate