On the reducible character of Haldane-Radić enzyme kinetics to conventional and logistic Michaelis-Menten models

Mihai V Putz

doi:10.3390/molecules16043128

On the reducible character of Haldane-Radić enzyme kinetics to conventional and logistic Michaelis-Menten models

Molecules. 2011 Apr 13;16(4):3128-45. doi: 10.3390/molecules16043128.

Author

Mihai V Putz¹

Affiliation

¹ Laboratory of Computational and Structural Physical Chemistry, Chemistry Department, West University of Timişoara, Pestalozzi Street No.16, Timisoara, RO-300115, Romania. mv_putz@yahoo.com

Abstract

The conceptual and practical issues regarding the reduction of the Haldane-Radić enzymic mechanism, specific for cholinesterase kinetics, to the consecrated or logistically modified Michaelis-Menten kinetics, specific for some mutant enzymes, are here clarified as due to the limited initial substrate concentration, through detailed initial rate and progress curve analysis, even when other classical conditions for such equivalence are not entirely fulfilled.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cholinesterases / genetics
Cholinesterases / metabolism*
Drosophila melanogaster
Hydrolysis
Kinetics
Logistic Models*
Mutation

Substances

Cholinesterases