The miRNA expression profile was initially established to investigate its corresponding function in human uveal melanoma. The miRNA expression profile in human uveal melanoma was analyzed by a micro chip technique. The hsa-miRNA expression between four uveal melanomas and four normal uveal tissues was compared. Based on the bioinformatic approach, chip data was analyzed to select out differentially expressed candidate hsa-miRNAs. Real-time quantitative PCR (RT-PCR) was used to confirm the candidate hsa-miRNAs expression in all samples. The results of miRNA microarray chips that matched with RT-PCR were considered as the miRNA expression which was significantly different between normal tissue and uveal melanomas. In four uveal melanomas, expressions of miRNA-20a, miRNA-106a, miRNA-17, miRNA-21, and miRNA-34a were significantly up-regulated, while miRNA-145 and miRNA-204 expression were significantly down-regulated. We used miRNA microarray analysis as a fast, efficient technology to study biological information. The differentially expressed miRNAs may be involved in uveal melanoma pathogenesis, and may help promote the diagnosis and treatment for uveal melanoma.