Abstract
High c-myc levels are linked to poor prognosis in medulloblastoma (MB), and it was the aim of the current study to search for c-myc-dependent proteins in the MB cell line D425Med. For this purpose D425Med cells and cells with knocked-down c-myc (by siRNA) were analysed by a gel-based differential proteomics study using mass spectrometry. Heterogeneous nuclear ribonucleoproteins C1/C2, heterogeneous nuclear ribonucleoprotein A/B, stathmin, endoplasmic reticulum protein ERp29 precursor and guanidinoacetate N-methyltransferase were c-myc dependently expressed. Signalling, the protein machinery, metabolism and endoplasmic reticulum function may be affected and these results enable studying tumour tissue for these proteins as potential dignity markers or pharmacological targets.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biomarkers, Tumor / genetics*
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Biomarkers, Tumor / metabolism
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Cell Line, Tumor
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Endoplasmic Reticulum / genetics
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Endoplasmic Reticulum / metabolism
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Gene Expression Regulation, Neoplastic*
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Gene Knockdown Techniques
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Guanidinoacetate N-Methyltransferase / genetics
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Guanidinoacetate N-Methyltransferase / metabolism
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Heat-Shock Proteins / genetics*
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Heat-Shock Proteins / metabolism
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Heterogeneous-Nuclear Ribonucleoproteins / genetics
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Heterogeneous-Nuclear Ribonucleoproteins / metabolism
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Humans
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Medulloblastoma / genetics*
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Medulloblastoma / metabolism
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Medulloblastoma / pathology
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Protein Precursors / genetics*
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Protein Precursors / metabolism
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Proto-Oncogene Proteins c-myc / genetics*
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Proto-Oncogene Proteins c-myc / metabolism
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RNA, Small Interfering / genetics
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Signal Transduction / genetics
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Stathmin / genetics
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Stathmin / metabolism
Substances
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Biomarkers, Tumor
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ERP29 protein, human
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Heat-Shock Proteins
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Heterogeneous-Nuclear Ribonucleoproteins
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Protein Precursors
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Proto-Oncogene Proteins c-myc
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RNA, Small Interfering
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STMN1 protein, human
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Stathmin
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Guanidinoacetate N-Methyltransferase