The fabrication, characterization and functionalization of periodically patterned nanoporous gold layers is presented. The material shows plasmonic properties in the near infrared range, with excitation and propagation of surface plasmon polaritons. Functionalization shows a marked enhancement in the optical response in comparison with evaporated gold gratings, due to a great increase of the active surface. Due to its superior response, nanoporous gold patterns appear promising for the realization of compact plasmonic platforms for sensing purposes.