Ting is a fermented sorghum product of Botswana which is used to prepare porridges of different consistencies. This study aimed to characterise ting with respect to fermentation microbiota and metabolite composition, and to develop starter cultures for ting fermentation. The pH values of 10 ting samples from Botswana ranged between 3.5 and 4.0 and cell counts ranged between 1.2×10(8) and 1.2×10(10)cfu/g lactic acid bacteria. Yeast cell counts were below 10(5)cfu/g in all samples and Enterobacteriaceae were less than 10 cfu/g. The microbiota of samples consisted of 2-4 dominant strains and strains were identified based on the sequence of their 16S rRNA genes as Lactobacillus reuteri, Lactobacillus fermentum, Lactobacillus harbinensis, Lactobacillus plantarum, Lactobacillus parabuchneri, Lactobacillus casei and Lactobacillus coryniformis. Binary strain combinations were employed as starter cultures to produce ting. Major fermentation products were lactate, acetate and ethanol, additionally, 1,2 propanediol and 1,3 propanediol were produced by some strains. All strains were capable of producing ting, which was accompanied by a decrease in pH from 6.3 to 4 within 8h. Traditionally processed ting requires 2 to 3 days to attain a pH below 4; the starter cultures thus reduced the fermentation time to 8h.
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