Abstract
The main aim of this research is to study the in vitro photocytotoxicity and cellular uptake of biodegradable polymeric nanoparticles loaded with photosensitizer mTHPP. As the first part of a continued research on conversion of N-sulfonato-N,O-carboxymethylchitosan (NOCCS) to useful biopolymer-based materials, large numbers of carboxylic functional groups were introduced onto NOCCS by grafting with polymethacrylic acid (PMAA). The free radical graft copolymerization was carried out at 70°C, bis-acrylamide as a cross-linking agent and persulfate as an initiator. These results show that the nanoparticles have high loading capacity and stability. These nanoparticles are suitable as carriers for photodynamic therapy in vivo.
Crown Copyright © 2011. Published by Elsevier B.V. All rights reserved.
MeSH terms
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Biocompatible Materials / chemical synthesis
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Biocompatible Materials / pharmacology
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Carcinoma / drug therapy*
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Carcinoma / pathology
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Cell Line, Tumor
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Cell Survival / drug effects
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Cell Survival / radiation effects
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Chitin / analogs & derivatives*
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Chitin / chemistry
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Cross-Linking Reagents / chemistry
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Drug Carriers / chemical synthesis
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Drug Carriers / pharmacology
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Drug Stability
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Humans
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Light
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Microscopy, Electron, Scanning
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Nanomedicine / methods*
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Nanoparticles / chemistry
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Nanoparticles / ultrastructure
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Neoplasms / drug therapy*
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Neoplasms / pathology
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Photochemotherapy / methods*
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Photosensitizing Agents / pharmacology*
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Polymethacrylic Acids / chemistry
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Porphyrins / pharmacology*
Substances
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Biocompatible Materials
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Cross-Linking Reagents
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Drug Carriers
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N-sulfonato-N,O-carboxymethylchitosan
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Photosensitizing Agents
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Polymethacrylic Acids
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Porphyrins
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tetra(3-hydroxyphenyl)porphine
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Chitin
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polymethacrylic acid