Abstract
A bi-layered silicon-releasable membrane consisting of a siloxane-poly(lactic acid) (PLA)-vaterite hybrid material (Si-PVH) microfiber mesh and a PLA microfiber mesh has been developed by an electrospinning method for guided bone regeneration (GBR) application. The bi-layered membrane was modified to a three-laminar structure by sandwiching an additional PLA microfiber mesh between the Si-PVH and PLA microfiber meshes (Si-PVH/PLA membrane). In this study, the influence of gamma irradiation, used for sterilization, on biological properties of the Si-PVH/PLA membrane was evaluated with osteoblasts and fibroblasts. After gamma irradiation, while the average molecular weight of the Si-PVH/PLA membrane decreased, the Si-PVH/PLA membrane promoted cell proliferation and differentiation (alkaline phosphatase activity and calcification) of osteoblasts, compared with the poly(lactide-co-glycolide) membrane. These results suggest that the gamma-irradiated Si-PVH/PLA membrane is biocompatible with both fibroblasts and osteoblasts, and may have an application for GBR.
MeSH terms
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3T3 Cells
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Alkaline Phosphatase / analysis
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Animals
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Biocompatible Materials / chemistry*
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Biocompatible Materials / radiation effects
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Bone Regeneration / physiology*
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Calcification, Physiologic / physiology
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Calcium Carbonate / chemistry*
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Calcium Carbonate / radiation effects
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Cell Culture Techniques
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Cell Differentiation / physiology
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Cell Proliferation
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Cells, Cultured
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Equipment Design
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Fibroblasts / physiology
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Gamma Rays
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Gingiva / cytology
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Guided Tissue Regeneration / instrumentation*
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Humans
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Lactic Acid / chemistry*
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Lactic Acid / radiation effects
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Materials Testing
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Membranes, Artificial*
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Mice
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Microscopy, Electron, Scanning
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Molecular Weight
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Osteoblasts / physiology
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Polyesters
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Polyglycolic Acid / chemistry
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Polyglycolic Acid / radiation effects
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Polylactic Acid-Polyglycolic Acid Copolymer
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Polymers / chemistry*
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Polymers / radiation effects
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Siloxanes / chemistry*
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Siloxanes / radiation effects
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Sterilization / methods
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Surgical Mesh
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X-Ray Diffraction
Substances
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Biocompatible Materials
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Membranes, Artificial
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Polyesters
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Polymers
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Siloxanes
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Polylactic Acid-Polyglycolic Acid Copolymer
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Polyglycolic Acid
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Lactic Acid
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poly(lactide)
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Alkaline Phosphatase
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Calcium Carbonate