Abstract
Macrolactam antibiotics are an important class of macrocyclic polyketides that contain a unique nitrogen-containing starter unit. In the present study, a set of starter biosynthetic enzymes in the macrolactam antibiotic vicenistatin was characterized. We found that the protection-deprotection strategy of the aminoacyl-ACP intermediate was critical in this system. On the basis of bioinformatics, the described pathway is also proposed as a common method for carrying amino acids in the biosynthesis of other macrolactam antibiotics.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acids / chemistry*
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Aminoglycosides / biosynthesis*
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Aminoglycosides / chemistry
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Anti-Bacterial Agents / biosynthesis*
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Anti-Bacterial Agents / chemistry
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Carboxy-Lyases / chemistry
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Carboxy-Lyases / metabolism*
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Intramolecular Transferases / chemistry
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Intramolecular Transferases / metabolism*
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Lactams / chemistry
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Ligases / chemistry
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Ligases / metabolism*
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Macrolides / chemistry
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Molecular Conformation
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Stereoisomerism
Substances
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Amino Acids
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Aminoglycosides
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Anti-Bacterial Agents
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Lactams
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Macrolides
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vicenistatin
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Carboxy-Lyases
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Intramolecular Transferases
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methylaspartate mutase
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Ligases