An epifluorescence microscopy method for generalized polarization imaging

Jesper Søndergaard Hansen; Claus Hélix-Nielsen

doi:10.1016/j.bbrc.2011.10.138

An epifluorescence microscopy method for generalized polarization imaging

Biochem Biophys Res Commun. 2011 Dec 2;415(4):686-90. doi: 10.1016/j.bbrc.2011.10.138. Epub 2011 Nov 6.

Authors

Jesper Søndergaard Hansen¹, Claus Hélix-Nielsen

Affiliation

¹ The Biomimetic Membrane Group, DTU Physics, Technical University of Denmark, Fysikvej 309, DK-2800 Kgs. Lyngby, Denmark. jsha@fysik.dtu.dk

PMID: 22079294
DOI: 10.1016/j.bbrc.2011.10.138

Abstract

Generalized polarization (GP) microscopy represents an excellent tool to study lipid-lipid and lipid-protein interactions in situ and in vitro. Here, we present an efficient and cost effective method to perform GP microscopy using a standard light-emitting diode (LED) epifluorescence microscope equipped with a digital color camera.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chick Embryo
Cost-Benefit Analysis
Fluorescence Polarization / economics
Fluorescence Polarization / methods*
Microscopy, Fluorescence / economics
Microscopy, Fluorescence / methods*
Microscopy, Polarization / economics
Microscopy, Polarization / methods*