Abstract
Clonal isolates identified as various nonfermentative Gram-negative bacilli over a 5-year period from sputum cultures of a 30-year-old cystic fibrosis patient were successfully reidentified as Pandoraea sputorum by combining 16S rRNA sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Decreased lung function improved after 1 year of azithromycin and inhaled 7%-hypertonic saline treatment.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Anti-Bacterial Agents / therapeutic use
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Azithromycin / therapeutic use
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Burkholderiaceae / chemistry
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Burkholderiaceae / classification*
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Burkholderiaceae / genetics
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Burkholderiaceae / isolation & purification*
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Cluster Analysis
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Cystic Fibrosis / complications*
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DNA Fingerprinting
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / genetics
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Electrophoresis, Gel, Pulsed-Field
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Female
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Gram-Negative Bacterial Infections / diagnosis*
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Gram-Negative Bacterial Infections / drug therapy
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Gram-Negative Bacterial Infections / microbiology*
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Humans
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Lung / physiopathology
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Mass Spectrometry
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Molecular Sequence Data
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Phylogeny
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RNA, Ribosomal, 16S / genetics
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Respiratory Function Tests
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Sequence Analysis, DNA
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Sputum / microbiology
Substances
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Anti-Bacterial Agents
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DNA, Bacterial
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DNA, Ribosomal
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RNA, Ribosomal, 16S
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Azithromycin