A study on neuroinflammatory marker in brain areas of okadaic acid (ICV) induced memory impaired rats

Life Sci. 2012 May 22;90(19-20):713-20. doi: 10.1016/j.lfs.2012.03.012. Epub 2012 Mar 26.

Abstract

Aims: The aim of the present study is to investigate the status of proinflammatory cytokine in the brain of intracerebroventricular (i.c.v.) okadaic acid (OKA) induced memory impaired rat.

Main methods: OKA (200 ng) intracerebroventricular (i.c.v.) was administered in rats. Memory was assessed by Morris water maze test. Biochemical marker of neuroinflammation (TNF-α, IL-β), total nitrite, mRNA (RT PCR) and protein expression (WB) of iNOS and nNOS were estimated in rat brain areas.

Key findings: OKA caused memory-impairment in rats with increased expression of proinflammatory cytokine TNF-α and IL-1β and total nitrite in brain regions hippocampus and cortex. The expression of mRNA and protein of iNOS was increased while; the expressions were decreased in case of nNOS. Pretreatment with antidementic drugs donepezil (5 mg/kg, p.o.) and memantine (10 mg/kg, p.o) for 13 days protected i.c.v. OKA induced memory impairment and changes in level of TNF-α, IL-β, total nitrite and expressions of iNOS and nNOS in OKA treated rat.

Significance: This study suggests that neuroinflammation may play a vital role in OKA induced memory impairment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Behavior, Animal / drug effects
  • Biomarkers
  • Blotting, Western
  • Donepezil
  • Excitatory Amino Acid Antagonists / pharmacology
  • Indans / pharmacology
  • Inflammation / chemically induced*
  • Inflammation / pathology*
  • Injections, Intraventricular
  • Interleukin-1beta / metabolism
  • Male
  • Maze Learning / drug effects
  • Memantine / pharmacology
  • Memory Disorders / chemically induced*
  • Memory Disorders / pathology*
  • Memory Disorders / psychology
  • Motor Activity / drug effects
  • Nitrate Reductase / metabolism
  • Nitric Oxide Synthase Type I / biosynthesis
  • Nitric Oxide Synthase Type II / biosynthesis
  • Nitrites / metabolism
  • Nootropic Agents / pharmacology
  • Okadaic Acid* / administration & dosage
  • Piperidines / pharmacology
  • Polymerase Chain Reaction
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Rats
  • Rats, Sprague-Dawley
  • Reactive Nitrogen Species / metabolism
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Biomarkers
  • Excitatory Amino Acid Antagonists
  • Indans
  • Interleukin-1beta
  • Nitrites
  • Nootropic Agents
  • Piperidines
  • RNA, Messenger
  • Reactive Nitrogen Species
  • Tumor Necrosis Factor-alpha
  • Okadaic Acid
  • Donepezil
  • Nitric Oxide Synthase Type I
  • Nitric Oxide Synthase Type II
  • Nitrate Reductase
  • Memantine