Synthesis of heparan sulfate tetrasaccharide as a substrate for human heparanase

Naoko Takeda; Remina Ikeda-Matsumi; Kaoru Ebara-Nagahara; Miyuki Otaki-Nanjo; Kayo Taniguchi-Morita; Miwa Nanjo; Jun-ichi Tamura

doi:10.1016/j.carres.2012.03.014

Synthesis of heparan sulfate tetrasaccharide as a substrate for human heparanase

Carbohydr Res. 2012 May 15:353:13-21. doi: 10.1016/j.carres.2012.03.014. Epub 2012 Mar 20.

Authors

Naoko Takeda¹, Remina Ikeda-Matsumi, Kaoru Ebara-Nagahara, Miyuki Otaki-Nanjo, Kayo Taniguchi-Morita, Miwa Nanjo, Jun-ichi Tamura

Affiliation

¹ Department of Regional Environment, Tottori University, Tottori 680-8551, Japan.

PMID: 22486828
DOI: 10.1016/j.carres.2012.03.014

Abstract

Regiospecifically sulfated heparan sulfate tetrasaccharide, GlcAβ-GlcN(NS6S)α-GlcAβ-GlcN(NS6S)α was first synthesized as an octyl glycoside. Total synthesis was achieved effectively by coupling the corresponding disaccharide units in short steps.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Sequence
Glucuronidase / metabolism*
Heparitin Sulfate / chemistry*
Humans
Molecular Sequence Data
Oligosaccharides / chemical synthesis*
Oligosaccharides / chemistry
Oligosaccharides / metabolism*
Substrate Specificity

Substances

Oligosaccharides
Heparitin Sulfate
heparanase
Glucuronidase