Deep 16S rRNA metagenomics and quantitative PCR analyses of the premature infant fecal microbiota

Silvia Arboleya; Li Ang; Abelardo Margolles; Li Yiyuan; Zhang Dongya; Xiao Liang; Gonzalo Solís; Nuria Fernández; Clara G de Los Reyes-Gavilán; Miguel Gueimonde

doi:10.1016/j.anaerobe.2012.04.013

Deep 16S rRNA metagenomics and quantitative PCR analyses of the premature infant fecal microbiota

Anaerobe. 2012 Jun;18(3):378-80. doi: 10.1016/j.anaerobe.2012.04.013. Epub 2012 May 8.

Authors

Silvia Arboleya¹, Li Ang, Abelardo Margolles, Li Yiyuan, Zhang Dongya, Xiao Liang, Gonzalo Solís, Nuria Fernández, Clara G de Los Reyes-Gavilán, Miguel Gueimonde

Affiliation

¹ Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, Villaviciosa, Spain.

PMID: 22579986
DOI: 10.1016/j.anaerobe.2012.04.013

Abstract

Metagenomic studies on the gut microbiota of preterm infants are scarce. We characterized the microbiota of 10 days-old neonates by deep 16S rRNA gene metagenomic analysis and compare the results with those obtained by qPCR. Both techniques lead to similar conclusions, allowing differentiating between preterm and full-term infants.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Bifidobacterium / genetics
Enterobacteriaceae / genetics
Feces / microbiology*
Female
High-Throughput Nucleotide Sequencing
Humans
Infant, Newborn
Infant, Premature*
Infant, Very Low Birth Weight
Lactobacillaceae / genetics
Male
Metagenome*
Metagenomics
Molecular Typing
RNA, Ribosomal, 16S / genetics*
Real-Time Polymerase Chain Reaction
Sequence Analysis, DNA
Streptococcaceae / genetics

Substances

RNA, Ribosomal, 16S