Enhanced detection of gold nanoparticles in agarose gel electrophoresis

Electrophoresis. 2012 Apr;33(8):1251-4. doi: 10.1002/elps.201100556.

Abstract

Gel electrophoresis is a powerful tool in gold nanoparticle (AuNP) research. While the technique is sensitive to the size, charge, and shape of particles, its optimal performance requires a relatively large amount of AuNP in the loading wells for visible detection of bands. We here describe a novel and more sensitive method for detecting AuNPs in agarose gels that involves staining the gel with the common organic fluorophore fluorescein, to produce AuNP band intensities that are linear with nanoparticle concentration and almost an order of magnitude larger than those obtained without staining the gel.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Electrophoresis, Agar Gel / methods*
  • Fluorescein / chemistry
  • Fluorescent Dyes / chemistry
  • Gold / analysis*
  • Gold / chemistry
  • Metal Nanoparticles / analysis*
  • Metal Nanoparticles / chemistry
  • Particle Size
  • Sensitivity and Specificity

Substances

  • Fluorescent Dyes
  • Gold
  • Fluorescein