Biodegradation of aniline in an alkaline environment by a novel strain of the halophilic bacterium, Dietzia natronolimnaea JQ-AN

Bioresour Technol. 2012 Aug:117:148-54. doi: 10.1016/j.biortech.2012.04.068. Epub 2012 Apr 26.

Abstract

Dietzia natronolimnaea JQ-AN was isolated from industrial wastewater containing aniline. Under aerobic conditions, the JQ-AN strain degraded 87% of the aniline in a 300 mg L(-1) aniline solution after 120 h of shake flask incubation in a medium containing sodium acetate. This strain had an unusually high salinity tolerance in minimal medium (0-6% NaCl, w/v). The optimal pH for microbial growth and aniline biodegradation was pH 8.0. Two liters of simulated aniline wastewater was created in a reactor at pH 8.0 and 3% NaCl (w/v), and biodegradation of aniline was tested over 7 days at 30 °C. For the initial concentrations of 100, 300, and 500 mg L(-1), 100%, 80.5% and 72% of the aniline was degraded, respectively. Strain JQ-AN may use an ortho-cleavage pathway for dissimilation of the catechol intermediate.

MeSH terms

  • Actinomycetales / drug effects*
  • Actinomycetales / genetics
  • Actinomycetales / growth & development
  • Actinomycetales / metabolism*
  • Alkalies / pharmacology*
  • Aniline Compounds / metabolism*
  • Base Sequence
  • Biodegradation, Environmental / drug effects
  • Bioreactors / microbiology
  • Hydrogen-Ion Concentration / drug effects
  • Metabolic Networks and Pathways / drug effects
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sodium Acetate / pharmacology
  • Sodium Chloride / pharmacology

Substances

  • Alkalies
  • Aniline Compounds
  • RNA, Ribosomal, 16S
  • Sodium Chloride
  • Sodium Acetate
  • aniline

Associated data

  • GENBANK/JN867323