Abstract
A cell-specific metabolic glycan labeling strategy has been developed using azidosugars encapsulated in ligand-targeted liposomes. The ligands are designed to bind specific cell-surface receptors that are only expressed or up-regulated in target cells, which mediates the intracellular delivery of azidosugars. The delivered azidosugars are metabolically incorporated into cell-surface glycans, which are then imaged via a bioorthogonal reaction.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Azides / administration & dosage
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Azides / analysis
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Azides / metabolism
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Carbohydrates / administration & dosage
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Carbohydrates / analysis
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Cell Line, Tumor
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Cell Membrane / metabolism*
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HeLa Cells
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Humans
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Lipid Metabolism
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Liposomes / chemistry
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Liposomes / metabolism*
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Polysaccharides / analysis
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Polysaccharides / metabolism*
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Staining and Labeling
Substances
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Azides
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Carbohydrates
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Liposomes
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Polysaccharides