Mitochondrial DNA variants influence mitochondrial bioenergetics in Drosophila melanogaster

C C Correa; W C Aw; R G Melvin; N Pichaud; J W O Ballard

doi:10.1016/j.mito.2012.06.005

Mitochondrial DNA variants influence mitochondrial bioenergetics in Drosophila melanogaster

Mitochondrion. 2012 Jul;12(4):459-64. doi: 10.1016/j.mito.2012.06.005. Epub 2012 Jun 24.

Authors

C C Correa¹, W C Aw, R G Melvin, N Pichaud, J W O Ballard

Affiliation

¹ School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney 2052, Australia.

PMID: 22735574
DOI: 10.1016/j.mito.2012.06.005

Abstract

The influence of mitochondrial DNA (mtDNA) mutations on human disease has been extensively studied, but the impact of mutations within the adaptive range is debated. We studied males from lines of Drosophila melanogaster that have a highly standardized nuclear genome but different mtDNA, at two ages. We measured mitochondrial respiration on permeabilized muscle fibers, hydrogen peroxide production of isolated mitochondria and mtDNA copy number of whole individuals. The results show that a small set of naturally occurring mtDNA mutations can have a significant influence on mitochondrial bioenergetics that may change as the organism ages.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Respiration
DNA, Mitochondrial / genetics*
Drosophila melanogaster / physiology*
Energy Metabolism*
Hydrogen Peroxide / metabolism
Male
Mitochondria / genetics*
Mitochondria / physiology*
Muscle Fibers, Skeletal / metabolism
Mutation*

Substances

DNA, Mitochondrial
Hydrogen Peroxide