Abstract
The chronological lifespan (CLS) of budding yeast is a model for the aging of post-mitotic cells in higher eukaryotes. We report here the development of a new method to assess yeast CLS. The new assay is simple, convenient and labor-saving. We applied this new method to screen natural compounds isolated from mushrooms and discovered beauveriolide I as a potent anti-aging agent.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Agaricales / chemistry*
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Cellular Senescence / drug effects*
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Cellular Senescence / physiology
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Cytochromes c1 / deficiency
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Cytochromes c1 / genetics
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Depsipeptides / chemistry
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Depsipeptides / isolation & purification
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Depsipeptides / pharmacology*
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Gene Expression
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High-Throughput Screening Assays*
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Histone Demethylases / deficiency
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Histone Demethylases / genetics
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Humans
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Life Expectancy
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Longevity
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Mitosis / drug effects
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Models, Biological
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Phosphatidylinositol 3-Kinases / deficiency
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Phosphatidylinositol 3-Kinases / genetics
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Saccharomyces cerevisiae / drug effects*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / genetics
Substances
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Depsipeptides
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Saccharomyces cerevisiae Proteins
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beauverolides
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Cytochromes c1
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GIS1 protein, S cerevisiae
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Histone Demethylases
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TOR1 protein, S cerevisiae