HyperCEST detection of a 129Xe-based contrast agent composed of cryptophane-A molecular cages on a bacteriophage scaffold

Magn Reson Med. 2013 May;69(5):1245-52. doi: 10.1002/mrm.24371. Epub 2012 Jul 12.

Abstract

A hyperpolarized 129Xe contrast agent composed of many cryptophane-A molecular cages assembled on an M13 bacteriophage has been demonstrated. Saturation of xenon bound in the large number of cryptophane cages is transferred to the pool of aqueous-solvated xenon via chemical exchange, resulting in efficient generation of hyperCEST contrast. No significant loss of contrast per cryptophane cage was observed for the multivalent phage when compared with unscaffolded cryptophane. Detection of this phage-based hyperCEST agent is reported at concentrations as low as 230 fM, representing the current lower limit for NMR/MRI-based contrast agents.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Algorithms
  • Bacteriophages / chemistry*
  • Biosensing Techniques / methods*
  • Contrast Media / analysis
  • Drug Carriers / chemistry*
  • Magnetic Resonance Spectroscopy / methods*
  • Molecular Imaging / methods*
  • Polycyclic Compounds / chemistry*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Xenon Isotopes / analysis*
  • Xenon Isotopes / chemistry

Substances

  • Contrast Media
  • Drug Carriers
  • Polycyclic Compounds
  • Xenon Isotopes
  • cryptophane A