Suppression of matrix ions by N-phosphorylation labeling using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry

Xiang Gao; Zhi Tang; Minghua Lu; Hongxia Liu; Yuyang Jiang; Yufen Zhao; Zongwei Cai

doi:10.1039/c2cc36091h

Suppression of matrix ions by N-phosphorylation labeling using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry

Chem Commun (Camb). 2012 Oct 21;48(82):10198-200. doi: 10.1039/c2cc36091h.

Authors

Xiang Gao¹, Zhi Tang, Minghua Lu, Hongxia Liu, Yuyang Jiang, Yufen Zhao, Zongwei Cai

Affiliation

¹ Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR, China.

PMID: 22962666
DOI: 10.1039/c2cc36091h

Abstract

N-Phosphorylation labeling was utilized to analyze small metabolites using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS). The incorporation of a neutral phosphoryl group with high gas-phase proton affinity not only improved the ionization efficiency of the target molecules, but also greatly reduced the matrix background interference.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alanine / analogs & derivatives
Alanine / analysis*
Alanine / chemical synthesis
Ions / analysis
Ions / chemical synthesis
Molecular Structure
Phosphorylation
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Staining and Labeling

Substances

Ions
Alanine