Abstract
A lysosome-specific and two-photon fluorescent probe, Lyso-NINO, demonstrates high selectivity and sensitivity toward NO, lower cytotoxicity, and perfect lysosomal localization. With the aid of Lyso-NINO, the first capture of NO within lysosomes of macrophage cells has been achieved using both two-photon fluorescence microscopy and flow cytometry.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Survival / drug effects
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Dose-Response Relationship, Drug
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Flow Cytometry
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Fluorescent Dyes / chemistry*
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Fluorescent Dyes / pharmacology
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Humans
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Lysosomes / chemistry*
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MCF-7 Cells
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Macrophages / chemistry*
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Macrophages / cytology
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Microscopy, Fluorescence
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Molecular Structure
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Morpholines / chemistry*
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Morpholines / pharmacology
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Naphthalimides / chemistry*
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Naphthalimides / pharmacology
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Nitric Oxide / analysis*
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Photons*
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Structure-Activity Relationship
Substances
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Fluorescent Dyes
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Lyso-NINO
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Morpholines
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Naphthalimides
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Nitric Oxide