A lysosome-targetable and two-photon fluorescent probe for monitoring endogenous and exogenous nitric oxide in living cells

J Am Chem Soc. 2012 Oct 24;134(42):17486-9. doi: 10.1021/ja308967u. Epub 2012 Oct 9.

Abstract

A lysosome-specific and two-photon fluorescent probe, Lyso-NINO, demonstrates high selectivity and sensitivity toward NO, lower cytotoxicity, and perfect lysosomal localization. With the aid of Lyso-NINO, the first capture of NO within lysosomes of macrophage cells has been achieved using both two-photon fluorescence microscopy and flow cytometry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Survival / drug effects
  • Dose-Response Relationship, Drug
  • Flow Cytometry
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / pharmacology
  • Humans
  • Lysosomes / chemistry*
  • MCF-7 Cells
  • Macrophages / chemistry*
  • Macrophages / cytology
  • Microscopy, Fluorescence
  • Molecular Structure
  • Morpholines / chemistry*
  • Morpholines / pharmacology
  • Naphthalimides / chemistry*
  • Naphthalimides / pharmacology
  • Nitric Oxide / analysis*
  • Photons*
  • Structure-Activity Relationship

Substances

  • Fluorescent Dyes
  • Lyso-NINO
  • Morpholines
  • Naphthalimides
  • Nitric Oxide