Detection of oxacillin-susceptible mecA-positive Staphylococcus aureus isolates by use of chromogenic medium MRSA ID

V Anil Kumar; Katherin Steffy; Maitrayee Chatterjee; Madhan Sugumar; Kavitha R Dinesh; Anand Manoharan; Shamsul Karim; Raja Biswas

doi:10.1128/JCM.01040-12

Detection of oxacillin-susceptible mecA-positive Staphylococcus aureus isolates by use of chromogenic medium MRSA ID

J Clin Microbiol. 2013 Jan;51(1):318-9. doi: 10.1128/JCM.01040-12. Epub 2012 Nov 7.

Authors

V Anil Kumar¹, Katherin Steffy, Maitrayee Chatterjee, Madhan Sugumar, Kavitha R Dinesh, Anand Manoharan, Shamsul Karim, Raja Biswas

Affiliation

¹ Department of Microbiology, Amrita Institute of Medical Sciences, Ponekara, Kochi, Kerala, India. vanilkumar@aims.amrita.edu

Abstract

Reports of oxacillin-susceptible mecA-positive Staphylococcus aureus strains are on the rise. Because of their susceptibility to oxacillin and cefoxitin, it is very difficult to detect them by using routine phenotypic methods. We describe two such isolates that were detected by chromogenic medium and confirmed by characterization of the mecA gene element.

Publication types

Evaluation Study

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacterial Proteins / genetics*
Bacteriological Techniques / methods*
Cefoxitin / pharmacology
Chromogenic Compounds / metabolism
Culture Media / chemistry*
Humans
Methicillin-Resistant Staphylococcus aureus / drug effects
Methicillin-Resistant Staphylococcus aureus / genetics
Methicillin-Resistant Staphylococcus aureus / isolation & purification*
Oxacillin / pharmacology*
Penicillin-Binding Proteins
Staphylococcal Infections / microbiology

Substances

Anti-Bacterial Agents
Bacterial Proteins
Chromogenic Compounds
Culture Media
Penicillin-Binding Proteins
mecA protein, Staphylococcus aureus
Cefoxitin
Oxacillin