Clostridium carboxidivorans strain P7T recombinant formate dehydrogenase catalyzes reduction of CO(2) to formate

Apostolos Alissandratos; Hye-Kyung Kim; Hayden Matthews; James E Hennessy; Amy Philbrook; Christopher J Easton

doi:10.1128/AEM.02886-12

Clostridium carboxidivorans strain P7T recombinant formate dehydrogenase catalyzes reduction of CO(2) to formate

Appl Environ Microbiol. 2013 Jan;79(2):741-4. doi: 10.1128/AEM.02886-12. Epub 2012 Nov 9.

Authors

Apostolos Alissandratos¹, Hye-Kyung Kim, Hayden Matthews, James E Hennessy, Amy Philbrook, Christopher J Easton

Affiliation

¹ CSIRO Biofuels Research Cluster, Research School of Chemistry, Australian National University, Canberra, Australia.

Abstract

Recombinant formate dehydrogenase from the acetogen Clostridium carboxidivorans strain P7(T), expressed in Escherichia coli, shows particular activity towards NADH-dependent carbon dioxide reduction to formate due to the relative binding affinities of the substrates and products. The enzyme retains activity over 2 days at 4°C under oxic conditions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbon Dioxide / metabolism*
Cloning, Molecular
Clostridium / enzymology*
Clostridium / genetics
Enzyme Stability
Escherichia coli / genetics
Formate Dehydrogenases / chemistry
Formate Dehydrogenases / genetics
Formate Dehydrogenases / isolation & purification
Formate Dehydrogenases / metabolism*
Formates / metabolism*
Gene Expression
Kinetics
Oxidation-Reduction
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Recombinant Proteins / isolation & purification
Recombinant Proteins / metabolism
Temperature
Time Factors

Substances

Formates
Recombinant Proteins
formic acid
Carbon Dioxide
Formate Dehydrogenases