In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies

Sylvia May; Mirjam Andreasson-Ochsner; Zhikang Fu; Ying Xiu Low; Darren Tan; Hans-Peter M de Hoog; Sandra Ritz; Madhavan Nallani; Eva-Kathrin Sinner

doi:10.1002/anie.201204645

In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies

Angew Chem Int Ed Engl. 2013 Jan 7;52(2):749-53. doi: 10.1002/anie.201204645. Epub 2012 Nov 19.

Authors

Sylvia May¹, Mirjam Andreasson-Ochsner, Zhikang Fu, Ying Xiu Low, Darren Tan, Hans-Peter M de Hoog, Sandra Ritz, Madhavan Nallani, Eva-Kathrin Sinner

Affiliation

¹ Patterning and Fabrication, Institute of Materials Research and Engineering, Agency for Science, Technology and Research, Singapore, Singapore.

PMID: 23161746
DOI: 10.1002/anie.201204645

Abstract

The dopamine receptor D2 (DRD2), a G-protein coupled receptor is expressed into PBd(22)-PEO(13) and PMOXA(20)-PDMS(54)-PMOXA(20) block copolymer vesicles. The conformational integrity of the receptor is confirmed by antibody- and ligand-binding assays. Replacement of bound dopamine is demonstrated on surface-immobilized polymersomes, thus making this a promising platform for drug screening.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Drug Discovery
Humans
Ligands
Polymers / chemistry*
Polymers / metabolism*
Receptors, G-Protein-Coupled / chemistry*
Receptors, G-Protein-Coupled / metabolism*

Substances

Ligands
Polymers
Receptors, G-Protein-Coupled