Reprogramming human fibroblasts to pluripotency using modified mRNA

Nat Protoc. 2013 Mar;8(3):568-82. doi: 10.1038/nprot.2013.019. Epub 2013 Feb 21.

Abstract

Induced pluripotent stem (iPS) cells hold the potential to revolutionize regenerative medicine through their capacity to generate cells of diverse lineages for future patient-specific cell-based therapies. To facilitate the transition of iPS cells to clinical practice, a variety of technologies have been developed for transgene-free pluripotency reprogramming. We recently reported efficient iPS cell generation from human fibroblasts using synthetic modified mRNAs. Here we describe a stepwise protocol for the generation of modified mRNA-derived iPS cells from primary human fibroblasts, focusing on the critical parameters including medium choice, quality control, and optimization steps needed for synthesizing modified mRNAs encoding reprogramming factors and introducing these into cells over the course of 2-3 weeks to ensure successful reprogramming. The protocol described herein is for reprogramming of human fibroblasts to pluripotency; however, the properties of modified mRNA make it a powerful platform for protein expression, which has broad applicability in directed differentiation, cell fate specification and therapeutic applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Culture Techniques*
  • Cell Differentiation*
  • Cellular Reprogramming*
  • Fibroblasts / cytology*
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • RNA, Messenger / chemistry*
  • Transfection / methods

Substances

  • RNA, Messenger