Abstract
Microtubules (MT) outline and maintain the overall shape of cells and can reorganize cellular membranes to serve as sites of RNA virus replication. Here, we provide data on involvement of an MT-associated protein in infection of plants with a potyvirus, Potato virus A (PVA), representing the largest family of plant-infecting RNA viruses. Our results showed that helper-component proteinase (HCpro)-interacting protein 2 (HIP2) of potato (Solanum tuberosum) is an MT-associated protein similar to Arabidopsis SPR2. Virus-induced silencing of HIP2 in Nicotiana benthamiana resulted in a spiral-like growth phenotype, similar to the Arabidopsis spr2 mutant, and the spr2 phenotype in Arabidopsis was complemented with potato HIP2. HCpro of PVA interacted with HIP2 of potato and tobacco (Nicotiana tabacum). The interaction was detected by bimolecular fluorescence complementation in PVA-infected leaves on MT and MT intersections at the cell cortex. HIP2-HCpro interaction was determined by the C-proximal α-helix-rich domain of HIP2, whereas the N-proximal putative TOG domain and the central coiled-coil domain of HIP2 controlled HIP2 dimerization and binding to MT. Accumulation of PVA was significantly reduced in the HIP2-silenced leaves of N. benthamiana, which indicates that HIP2-HCpro interactions are important for virus infection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / genetics
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Arabidopsis / metabolism
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Arabidopsis / virology
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Arabidopsis Proteins / chemistry
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism
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Base Sequence
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Cysteine Endopeptidases / genetics
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Cysteine Endopeptidases / metabolism*
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Gene Knockout Techniques
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Gene Silencing
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Genetic Complementation Test
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Host-Pathogen Interactions*
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Microtubule-Associated Proteins / chemistry
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Microtubule-Associated Proteins / genetics
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Microtubule-Associated Proteins / metabolism*
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Microtubules / metabolism
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Molecular Sequence Data
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Nicotiana / genetics
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Nicotiana / immunology
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Nicotiana / metabolism*
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Nicotiana / virology
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Phenotype
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Phylogeny
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Plant Diseases / immunology
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Plant Diseases / virology
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Plant Leaves / genetics
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Plant Leaves / immunology
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Plant Leaves / metabolism
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Plant Leaves / virology
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Plant Proteins / chemistry
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Plant Proteins / genetics
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Plant Proteins / metabolism
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Plants, Genetically Modified
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Potyvirus / enzymology
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Potyvirus / pathogenicity
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Potyvirus / physiology*
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Protein Interaction Mapping
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Sequence Analysis, DNA
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Solanum tuberosum / genetics
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Solanum tuberosum / immunology
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Solanum tuberosum / metabolism
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Solanum tuberosum / virology
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Viral Proteins / genetics
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Viral Proteins / metabolism*
Substances
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Arabidopsis Proteins
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Microtubule-Associated Proteins
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Plant Proteins
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SPIRAL2 protein, Arabidopsis
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Viral Proteins
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Cysteine Endopeptidases
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HC-Pro protein, potyvirus
Associated data
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GENBANK/HE649915
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GENBANK/HE653966