Abstract
The KCTD family includes tetramerization (T1) domain containing proteins with diverse biological effects. We identified a novel member of the KCTD family, BTBD10. A comprehensive analysis of protein-protein interactions (PPIs) allowed us to put forth a number of testable hypotheses concerning the biological functions for individual KCTD proteins. In particular, we predict that KCTD20 participates in the AKT-mTOR-p70 S6k signaling cascade, KCTD5 plays a role in cytokinesis in a NEK6 and ch-TOG-dependent manner, KCTD10 regulates the RhoA/RhoB pathway. Developmental regulator KCTD15 represses AP-2α and contributes to energy homeostasis by suppressing early adipogenesis. TNFAIP1-like KCTD proteins may participate in post-replication DNA repair through PCNA ubiquitination. KCTD12 may suppress the proliferation of gastrointestinal cells through interference with GABAb signaling. KCTD9 deserves experimental attention as the only eukaryotic protein with a DNA-like pentapeptide repeat domain. The value of manual curation of PPIs and analysis of existing high-throughput data should not be underestimated.
© 2013 WILEY Periodicals, Inc.
MeSH terms
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Animals
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Cell Differentiation / genetics*
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DNA Replication
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Gene Expression Regulation, Developmental
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Humans
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Intracellular Signaling Peptides and Proteins
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NIMA-Related Kinases
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Nuclear Proteins / genetics
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Nuclear Proteins / physiology*
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Potassium Channels / genetics
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Potassium Channels / physiology
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Potassium Channels, Voltage-Gated / genetics
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Potassium Channels, Voltage-Gated / physiology
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Proliferating Cell Nuclear Antigen / genetics
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Proliferating Cell Nuclear Antigen / physiology
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / physiology
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Repressor Proteins / genetics
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Repressor Proteins / physiology*
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Ribosomal Protein S6 Kinases, 70-kDa / genetics
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Ribosomal Protein S6 Kinases, 70-kDa / physiology
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Signal Transduction
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TOR Serine-Threonine Kinases / genetics
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TOR Serine-Threonine Kinases / physiology
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Ubiquitination
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Vertebrates / genetics*
Substances
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BTBD10 protein, human
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Intracellular Signaling Peptides and Proteins
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KCTD10 protein, human
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KCTD15 protein, human
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KCTD5 protein, human
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KCTD9 protein, human
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Nuclear Proteins
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Potassium Channels
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Potassium Channels, Voltage-Gated
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Proliferating Cell Nuclear Antigen
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Repressor Proteins
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MTOR protein, human
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NEK6 protein, human
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NIMA-Related Kinases
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Protein Serine-Threonine Kinases
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Ribosomal Protein S6 Kinases, 70-kDa
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TOR Serine-Threonine Kinases