Crosslinked gelatin nanofibres: preparation, characterisation and in vitro studies using glial-like cells

C Tonda-Turo; E Cipriani; S Gnavi; V Chiono; C Mattu; P Gentile; I Perroteau; M Zanetti; G Ciardelli

doi:10.1016/j.msec.2013.02.039

Crosslinked gelatin nanofibres: preparation, characterisation and in vitro studies using glial-like cells

Mater Sci Eng C Mater Biol Appl. 2013 Jul 1;33(5):2723-35. doi: 10.1016/j.msec.2013.02.039. Epub 2013 Feb 28.

Authors

C Tonda-Turo¹, E Cipriani, S Gnavi, V Chiono, C Mattu, P Gentile, I Perroteau, M Zanetti, G Ciardelli

Affiliation

¹ Politecnico di Torino, Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, Torino, Italy.

PMID: 23623089
DOI: 10.1016/j.msec.2013.02.039

Abstract

Gelatin (GL) nanofibrous matrices mimicking the complex biological structure of the natural extracellular matrix (ECM) were prepared from aqueous solutions by electrospinning technique. GL nanofibres with a diameter size of around 300nm were obtained optimising the process and solution parameters. To increase the GL stability in aqueous environment γ-glycidoxypropyltrimethoxysilane (GPTMS) was used as GL crosslinker. GPTMS crosslinking did not modify the nanofibrous matrix morphology: fibre diameter and membrane pores size were 327±45 nm and 1.64±0.37 μm, respectively. The produced GPTMS crosslinked GL nanofibres (GL/GPTMS_NF) were found to support the in vitro adhesion, proliferation and survival of neonatal olfactory bulb ensheating cells (NOBECs).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Proliferation
Cells, Cultured
Cross-Linking Reagents / chemistry
Extracellular Matrix / metabolism
Gelatin / chemistry*
Gelatin / metabolism
In Vitro Techniques
Microscopy, Electron, Scanning
Nanofibers*
Neuroglia / cytology
Neuroglia / metabolism*
Rats
Solubility
Tissue Scaffolds

Substances

Cross-Linking Reagents
Gelatin