Abstract
Reduced graphene oxide (RGO) and silver nanoparticle (AgNP) hybrids (RGO-AgNP) were prepared by a facile one-pot method using Poly (N-vinyl-2-pyrrolidone) as reductant and stabilizer. Folic acid (FA) molecules were attached to the RGO-AgNP by physisorption for targeting specific cancer cells with folate receptors (FRs) and using as Raman reporter molecules. The internalization of the FA loaded RGO-AgNP (RGO-AgNP-FA) inside the FRs-positive cancer cell was confirmed by confocal laser scanning and transmission electron microscopy. The Raman signals of the FA in live cancer cells were detected by confocal Raman spectroscope at 514 nm excitation, indicating that the RGO-AgNP-FA material has great potential as a Raman probe for cancer diagnosis in vitro.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / diagnosis
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Adenocarcinoma / metabolism
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Biosensing Techniques / methods
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Coated Materials, Biocompatible / pharmacokinetics
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Coated Materials, Biocompatible / toxicity
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Female
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Folic Acid / pharmacokinetics*
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Graphite / chemical synthesis*
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Graphite / toxicity
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HeLa Cells
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Humans
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Lung Neoplasms / diagnosis
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Lung Neoplasms / metabolism
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Metal Nanoparticles / chemistry*
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Metal Nanoparticles / toxicity
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Microscopy, Electron, Transmission
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Oxides / chemical synthesis
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Oxides / toxicity
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Silver / chemistry*
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Silver / toxicity
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Spectrum Analysis, Raman / methods*
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Uterine Cervical Neoplasms / diagnosis*
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Uterine Cervical Neoplasms / metabolism
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Vitamin B Complex / pharmacokinetics
Substances
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Coated Materials, Biocompatible
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Oxides
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Vitamin B Complex
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Silver
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Graphite
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Folic Acid