Abstract
The Carba NP test was evaluated against a panel of 244 carbapenemase- and non-carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa isolates. We confirmed the 100% specificity and positive predictive value of the test, but the sensitivity and negative predictive value were 72.5% and 69.2%, respectively, and increased to 80% and 77.3%, respectively, using a more concentrated bacterial extract. False-negative results were associated with mucoid strains or linked to enzymes with low carbapenemase activity, particularly OXA-48-like, which has emerged globally in enterobacteria.
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / genetics
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Bacterial Proteins / isolation & purification*
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Enterobacteriaceae / drug effects
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Enterobacteriaceae / enzymology*
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Enterobacteriaceae / genetics
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Enterobacteriaceae / isolation & purification
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Humans
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Microbial Sensitivity Tests / standards*
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Pseudomonas aeruginosa / drug effects
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Pseudomonas aeruginosa / enzymology*
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Pseudomonas aeruginosa / genetics
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Pseudomonas aeruginosa / isolation & purification
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Sensitivity and Specificity
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beta-Lactamases / biosynthesis
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beta-Lactamases / genetics
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beta-Lactamases / isolation & purification*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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beta-Lactamases
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carbapenemase