A highly efficient colorimetric immunoassay using a nanocomposite entrapping magnetic and platinum nanoparticles in ordered mesoporous carbon

Adv Healthc Mater. 2014 Jan;3(1):36-41. doi: 10.1002/adhm.201300100. Epub 2013 Jul 5.

Abstract

Nanocomposite to achieve ultrafast immunoassay: a new synergistically integrated nanocomposite consisting of magnetic and platinum nanoparticles, simultaneously entrapped in mesoporous carbon, is developed as a promising enzyme mimetic candidate to achieve ultrafast colorimetric immunoassays. Using new assay system, clinically important target molecules, such as human epidermal growth factor receptor 2 (HER2) and diarrhea-causing rotavirus, can be detected in only 3 min at room temperature with high specificity and sensitivity.

Keywords: enzyme mimetics; immunoassays; magnetic nanoparticles; mesoporous materials; nanocomposite.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / immunology
  • Biocompatible Materials / chemistry
  • Biocompatible Materials / metabolism
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology
  • Carbon / chemistry
  • Colorimetry*
  • Female
  • Ferrosoferric Oxide / chemistry
  • Humans
  • Immunoassay / instrumentation
  • Immunoassay / methods*
  • MCF-7 Cells
  • Magnetite Nanoparticles / chemistry
  • Metal Nanoparticles / chemistry
  • Nanocomposites / chemistry*
  • Platinum / chemistry
  • Porosity
  • Receptor, ErbB-2 / analysis*
  • Receptor, ErbB-2 / immunology
  • Rotavirus / immunology
  • Rotavirus / isolation & purification*
  • Temperature
  • Time Factors

Substances

  • Antibodies
  • Biocompatible Materials
  • Magnetite Nanoparticles
  • Platinum
  • Carbon
  • ERBB2 protein, human
  • Receptor, ErbB-2
  • Ferrosoferric Oxide