Beneficial effect of rutin on oxonate-induced hyperuricemia and renal dysfunction in mice

Pharmacology. 2013;92(1-2):75-83. doi: 10.1159/000351703. Epub 2013 Aug 10.

Abstract

Aims: The aim of the present study was to investigate the effects of rutin on potassium oxonate-induced hyperuricemia and renal dysfunction in mice.

Methods: Rutin was administered orally 1 h after oxonate at doses of 25, 50 and 100 mg·kg(-1). Serum uric acid levels and kidney function parameters were assayed. Mouse uromodulin levels in serum, urine and kidney were determined by the ELISA method. Simultaneously, the expression levels of renal organic ion transporters were detected.

Results: Rutin significantly decreased serum urate, creatinine and blood urea nitrogen, serum and kidney uromodulin levels, and increased urine uromodulin, urate and creatinine excretion in hyperuricemic mice. Rutin at 50 and 100 mg·kg(-1) significantly downregulated mRNA and protein levels of mouse glucose transporter 9 and urate transporter 1, and upregulated mRNA and protein levels of organic anion transporter 1 and organic cation/carnitine transporters in the kidney of hyperuricemic mice.

Conclusions: Rutin exerted its hypouricemic action and renal function improvement by the regulation of renal organic ion transporters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carrier Proteins / metabolism
  • Glucose Transport Proteins, Facilitative / genetics
  • Glucose Transport Proteins, Facilitative / metabolism
  • Hyperuricemia / chemically induced
  • Hyperuricemia / drug therapy*
  • Hyperuricemia / metabolism
  • Kidney Diseases / chemically induced
  • Kidney Diseases / drug therapy*
  • Kidney Diseases / metabolism
  • Male
  • Membrane Proteins / metabolism
  • Mice
  • Octamer Transcription Factor-1 / metabolism
  • Organic Anion Transport Protein 1 / genetics
  • Organic Anion Transport Protein 1 / metabolism
  • Organic Anion Transporters / genetics
  • Organic Anion Transporters / metabolism
  • Organic Cation Transport Proteins / metabolism
  • Organic Cation Transporter 2
  • Oxonic Acid
  • RNA, Messenger / metabolism
  • Rutin / pharmacology
  • Rutin / therapeutic use*
  • Solute Carrier Family 22 Member 5
  • Symporters
  • Uromodulin / genetics
  • Uromodulin / metabolism

Substances

  • Carrier Proteins
  • Glucose Transport Proteins, Facilitative
  • Membrane Proteins
  • Octamer Transcription Factor-1
  • Organic Anion Transport Protein 1
  • Organic Anion Transporters
  • Organic Cation Transport Proteins
  • Organic Cation Transporter 2
  • Pou2f1 protein, mouse
  • RNA, Messenger
  • Slc22a12 protein, mouse
  • Slc22a2 protein, mouse
  • Slc22a4 protein, mouse
  • Slc22a5 protein, mouse
  • Slc22a6 protein, mouse
  • Slc2a9 protein, mouse
  • Solute Carrier Family 22 Member 5
  • Symporters
  • Uromodulin
  • Rutin
  • Oxonic Acid