Ultrasound-mediated drug delivery using liposomes modified with a thermosensitive polymer

Ultrason Sonochem. 2014 Jan;21(1):310-6. doi: 10.1016/j.ultsonch.2013.07.014. Epub 2013 Aug 1.

Abstract

Ultrasound-mediated drug delivery was established using liposomes that were modified with the thermosensitive polymer (TSP) poly(NIPMAM-co-NIPAM), which sensitized the liposomes to high temperatures. TSP-modified liposomes (TSP liposomes) released encapsulated calcein under 1 MHz ultrasound irradiation at 0.5 W/cm(2) for 120 s as well as the case under incubation at 42 °C for 15 min. In addition, uptake of the drug released from TSP liposomes by cancer cells was enhanced by ultrasound irradiation. In a cell injury assay using doxorubicin (DOX)-loaded TSP liposomes and ultrasound irradiation, cell viability of HepG2 cells at 6 h after ultrasound irradiation (1 MHz, 0.5 W/cm(2) for 30 s) with DOX-loaded TSP liposomes (TSP/lipid ratio=1) was 60%, which was significantly lower than that of the control conditions such as DOX-loaded TSP liposomes alone and DOX-loaded intact liposomes under ultrasound irradiation.

Keywords: Cavitation; Drug delivery; Temperature-sensitive liposomes; Thermosensitive polymer; Ultrasound.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylic Resins / chemistry*
  • Biological Transport
  • Drug Delivery Systems / methods*
  • Hep G2 Cells
  • Humans
  • Liposomes / chemistry*
  • Polymers / chemistry*
  • Temperature*
  • Ultrasonics*

Substances

  • Acrylic Resins
  • Liposomes
  • Polymers
  • poly(N-isopropylmethacrylamide-co-N-isopropylacrylamide)