Ultrasensitive detection of small molecule-protein interaction via terminal protection of small molecule linked DNA and Exo III-aided DNA recycling amplification

Guohua Zhou; Xian Zhang; Xinghu Ji; Zhike He

doi:10.1039/c3cc43621g

Ultrasensitive detection of small molecule-protein interaction via terminal protection of small molecule linked DNA and Exo III-aided DNA recycling amplification

Chem Commun (Camb). 2013 Oct 9;49(78):8854-6. doi: 10.1039/c3cc43621g.

Authors

Guohua Zhou¹, Xian Zhang, Xinghu Ji, Zhike He

Affiliation

¹ Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China. xhu@whu.edu.cn zhkhe@whu.edu.cn.

PMID: 23964359
DOI: 10.1039/c3cc43621g

Abstract

Terminal protection of small molecule linked DNA assays amplified by Exo III-aided DNA recycling is developed for the detection of streptavidin-biotin interaction, with a detection limit of 0.8 fM streptavidin in the "turn-on" state, indicating that the proposed method is an ultrasensitive platform for the detection of small molecule-protein interactions.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques / methods*
Biotin / metabolism*
DNA / chemistry
DNA / metabolism*
Exodeoxyribonucleases / chemistry
Exodeoxyribonucleases / metabolism*
Limit of Detection
Nucleic Acid Amplification Techniques
Protein Binding
Streptavidin / analysis
Streptavidin / metabolism*

Substances

Biotin
DNA
Streptavidin
Exodeoxyribonucleases
exodeoxyribonuclease III