High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles

Fabrizio Chiodo; Marco Marradi; Boris Tefsen; Harm Snippe; Irma van Die; Soledad Penadés

doi:10.1371/journal.pone.0073027

High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles

PLoS One. 2013 Aug 27;8(8):e73027. doi: 10.1371/journal.pone.0073027. eCollection 2013.

Authors

Fabrizio Chiodo¹, Marco Marradi, Boris Tefsen, Harm Snippe, Irma van Die, Soledad Penadés

Affiliation

¹ Laboratory of GlycoNanotechnology, Biofunctional Nanomaterials Unit, CIC biomaGUNE, San Sebastián, Spain.

Abstract

Improved detection of anti-carbohydrate antibodies is a need in clinical identification of biomarkers for cancer cells or pathogens. Here, we report a new ELISA approach for the detection of specific immunoglobulins (IgGs) against carbohydrates. Two nanometer gold glyconanoparticles bearing oligosaccharide epitopes of HIV or Streptococcus pneumoniae were used as antigens to coat ELISA-plates. A ~3,000-fold improved detection of specific IgGs in mice immunized against S. pneumoniae respect to the well known BSA-glycoconjugate ELISA was achieved. Moreover, these multivalent glyconanoparticles have been employed in solid phase assays to detect the carbohydrate-dependent binding of human dendritic cells and the lectin DC-SIGN. Multivalent glyconanoparticles in ELISA provide a versatile, easy and highly sensitive method to detect and quantify the binding of glycan to proteins and to facilitate the identification of biomarkers.

Publication types

Clinical Trial
Research Support, Non-U.S. Gov't

MeSH terms

AIDS Vaccines / immunology
Animals
Antibodies, Bacterial / chemistry*
Antibodies, Bacterial / immunology
Epitopes / chemistry*
Epitopes / immunology
Female
HIV Antibodies / chemistry*
HIV Antibodies / immunology
HIV-1* / chemistry
HIV-1* / immunology
Humans
Male
Mice
Nanoparticles / chemistry*
Polysaccharides, Bacterial / chemistry*
Polysaccharides, Bacterial / immunology
Streptococcal Vaccines / immunology
Streptococcus pneumoniae* / chemistry
Streptococcus pneumoniae* / immunology

Substances

AIDS Vaccines
Antibodies, Bacterial
Epitopes
HIV Antibodies
Polysaccharides, Bacterial
Streptococcal Vaccines

Grants and funding

This research was supported by the Spanish Ministry of Economy and Competitiveness, (grant CTQ2011-04638), and the Department of Industry of the Basque Country (grant ETORTEK biomaGUNE 2011). The authors thank the COST action CM1102 for support. FC thanks Ministerio de Ciencia e Innovacion for a predoctoral grant. BT was financially supported by the Dutch Technology Foundation (STW, grant 10622). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.