In this study, detection of staphylococcal enterotoxin A (SEA) in multi-matrices using a highly sensitive and specific microplate chemiluminescence enzyme immunoassay (CLEIA) has been established. A pair of monoclonal antibodies (mAbs) was selected from 37 anti-SEA mAbs by pairwise analysis, and the experimental conditions of the CLEIA were optimized. This CLEIA exhibited high performance with a wide dynamic range from 6.4 pg mL(-1) to 1600 pg mL(-1), and the measured low limit of detection (LOD) was 3.2 pg mL(-1). No cross-reactivity was observed when this method was applied to test SEB, SEC1, and SED. It has also been successfully applied for analyzing SEA in a variety of environmental, biological, and clinical matrices, such as sewage, tap water, river water, roast beef, peanut butter, cured ham, 10% nonfat dry milk, milk, orange juice, human urine, and serum. Thus, the highly sensitive and SEA-specific CLEIA should make it attractive for quantifying SEA in public health and diagnosis in near future.
Keywords: CLEIA; Chemiluminescence enzyme immunoassay; Detection; Diagnosis; LOD; SEA; SEs; SFP; Sensitivity; Staphylococcal enterotoxin A; chemiluminescence enzyme immunoassay; detection of staphylococcal enterotoxin A; low limit of detection; mAbs; monoclonal antibodies; staphylococcal enterotoxins; staphylococcal food poisoning.
Crown Copyright © 2013. Published by Elsevier B.V. All rights reserved.