Light it up: human chromosome 7 ORF 24, a tumor-related protein, has been identified as a γ-glutamyl cyclotransferase (GGCT) in the glutathione homeostasis cycle. The singular substrate preference of the enzyme has hampered chemical probe development, and no fluorogenic probe has been reported. Here we report the first fluorogenic dipeptide probe, LISA-4, which should contribute toward further understanding of GGCT.
Keywords: O-to-N acyl migration; activity-based probes; cancer; fluorescent probes; gamma-glutamyl cyclotransferase.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.