A multiplexed nucleic acid microsystem for point-of-care detection of HIV co-infection with MTB and PCP

Talanta. 2013 Dec 15:117:532-5. doi: 10.1016/j.talanta.2013.08.056. Epub 2013 Sep 16.

Abstract

Many individuals infected with the human immunodeficiency virus (HIV), especially children in African countries, die of co-infections with Mycobacterium tuberculosis (MTB) (coinfection rate: 50%) or Pneumocystis carinii pneumonia (PCP) (coinfection rate: 81%). The present proposal describes a rapid, portable, low-cost, multiplexed point-of-care diagnostic technique for simultaneously detecting HIV, MTB, and PCP. This technique incorporates a creative micro-device (hardware) and a loop-mediated isothermal amplification strategy (software).

Keywords: Human immunodeficiency virus (HIV); Loop-mediated isothermal amplification (LAMP); M. tuberculosis (MTB); Microfluidics; Pneumocystis carinii pneumonia (PCP).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Child
  • Coinfection
  • DNA Primers / chemistry
  • DNA, Bacterial / genetics*
  • DNA, Fungal / genetics*
  • DNA, Viral / genetics*
  • HIV Infections / diagnosis*
  • HIV Infections / virology
  • Humans
  • Microfluidic Analytical Techniques / economics
  • Microfluidic Analytical Techniques / instrumentation
  • Microfluidic Analytical Techniques / methods*
  • Nucleic Acid Amplification Techniques
  • Plasmids / chemistry
  • Pneumonia, Pneumocystis / diagnosis*
  • Pneumonia, Pneumocystis / microbiology
  • Point-of-Care Systems
  • Sensitivity and Specificity
  • Tuberculosis, Pulmonary / diagnosis*
  • Tuberculosis, Pulmonary / microbiology

Substances

  • DNA Primers
  • DNA, Bacterial
  • DNA, Fungal
  • DNA, Viral