Amidase encapsulated O-carboxymethyl chitosan nanoparticles for vaccine delivery

Int J Biol Macromol. 2014 Feb:63:154-7. doi: 10.1016/j.ijbiomac.2013.10.045. Epub 2013 Nov 5.

Abstract

This work reports the development of amidase encapsulated O-carboxymethyl chitosan nanoparticles (Ami-O-CMC NPs) of 300±50 nm size by ionic cross-linking method. The prepared Ami-O-CMC NPs had an encapsulation efficiency of 55.39%. Haemolysis assay and cytotoxicity studies proved the hemocompatibility and cytocompatibility of the prepared NPs. The sustained release of Ami from the NPs is expected to prolong its immunogenicity and in turn lead to development of better protective immunity against Staphylococcus aureus infections.

Keywords: Amidase encapsulation; Immunogenicity; Staphylococcus aureus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amidohydrolases / chemistry*
  • Chitosan / administration & dosage
  • Chitosan / analogs & derivatives*
  • Chitosan / chemistry
  • Drug Delivery Systems*
  • Humans
  • Immunotherapy, Active
  • Nanoparticles / administration & dosage
  • Nanoparticles / chemistry
  • Staphylococcal Infections / drug therapy
  • Staphylococcal Infections / microbiology
  • Staphylococcal Infections / pathology
  • Staphylococcus aureus / drug effects*

Substances

  • O-carboxymethylchitosan
  • Chitosan
  • Amidohydrolases
  • amidase