Knockdown of the gene for homeobox protein HOXB13 reduces toxicity of oxidative-stress inducers in HEK293 cells

J Toxicol Sci. 2013;38(6):821-2. doi: 10.2131/jts.38.821.

Abstract

HEK293 cells transfected with a double-stranded siRNA to suppress expression of the homeobox gene HOXB13 were highly resistant to oxidative stress-inducing agents, such as hydrogen peroxide, N-ethylmaleimide (NEM), and paraquat. This finding suggests that HOXB13 might enhance cell injury caused by oxidative stress.

MeSH terms

  • Cell Death / drug effects*
  • Cell Death / genetics*
  • Ethylmaleimide / toxicity*
  • Gene Expression / genetics
  • Gene Knockdown Techniques*
  • HEK293 Cells
  • Homeodomain Proteins / genetics*
  • Homeodomain Proteins / physiology*
  • Humans
  • Hydrogen Peroxide / toxicity*
  • Oxidative Stress / drug effects*
  • Oxidative Stress / genetics*
  • Oxidative Stress / physiology
  • Paraquat / toxicity*
  • RNA, Double-Stranded
  • RNA, Small Interfering

Substances

  • HOXB13 protein, human
  • Homeodomain Proteins
  • RNA, Double-Stranded
  • RNA, Small Interfering
  • Hydrogen Peroxide
  • Ethylmaleimide
  • Paraquat