Novel class of rDNA repeat units in somatic hybrids between Nicotiana and Atropa

N V Borisjuk; V P Momot; Y Gleba

doi:10.1007/BF00288839

Novel class of rDNA repeat units in somatic hybrids between Nicotiana and Atropa

Theor Appl Genet. 1988 Jul;76(1):108-12. doi: 10.1007/BF00288839.

Authors

N V Borisjuk¹, V P Momot, Y Gleba

Affiliation

¹ Institute of Botany, Academy of Sciences of the Ukrainian SSR, Repina 2, 252601, Kiev, USSR.

PMID: 24231990
DOI: 10.1007/BF00288839

Abstract

Behavior of ribosomal RNA genes in the process of somatic hybridization was analyzed using hybrids Nicotiana tabacum + Atropa belladonna. Blothybridization of parental species DNAs to (32)P-rDNA specific probes revealed two classes of ribosomal repeats in both tobacco and nightshade; their length was 11.2 kb, 10.4 kb (tobacco) and 9.4 kb, 10.2 kb (night-shade). For analysis of hybrids, labelled (32)P rDNA specific probes were hybridized to DNA of parental species and somatic hybrids digested with restriction endonucleases EcoR1, EcoRV and BamH1. A new class of ribosomal DNA repeat, absent in parental species, was found in hybrid line NtAb-1. Possible mechanisms of appearence of a new rDNA class in the process of somatic cell fusion are discussed.