The development of an experimental multiple serogroups vaccine for Neisseria meningitidis

Valerian B Pinto; Robert Burden; Allyn Wagner; Elizabeth E Moran; Che-Hung Lee

doi:10.1371/journal.pone.0079304

The development of an experimental multiple serogroups vaccine for Neisseria meningitidis

PLoS One. 2013 Nov 14;8(11):e79304. doi: 10.1371/journal.pone.0079304. eCollection 2013.

Authors

Valerian B Pinto¹, Robert Burden, Allyn Wagner, Elizabeth E Moran, Che-Hung Lee

Affiliation

¹ Division of Bacterial and Rickettsial Diseases, Walter Reed Army Institute of Research (WRAIR), Silver Springs, Maryland, United States of America.

Abstract

A native outer membrane vesicles (NOMV) vaccine was developed from three antigenically diverse strains of Neisseria meningitidis that express the L1,8, L2, and L3,7 lipooligosaccharide (LOS) immunotypes, and whose synX, and lpxL1 genes were deleted.. Immunogenicity studies in mice showed that the vaccine induced bactericidal antibody against serogroups B, C, W, Y and X N. meningitidis strains. However, this experimental NOMV vaccine was not effective against serogroup A N. meningitidis strains. N. meningitidis capsular polysaccharide (PS) from serogroups A, C, W and Y were effective at inducing bactericidal antibody when conjugated to either tetanus toxoid or the fHbp1-fHbp2 fusion protein fHbp(1+2). The combination of the NOMV vaccine and the N. meningitidis serogroup A capsular polysaccharide (MAPS) protein conjugate was capable of inducing bactericidal antibodies against a limited number of N. meningitidis strains from serogroups A, B, C, W, Y and X tested in this study.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens, Bacterial / genetics
Antigens, Bacterial / immunology
Bacterial Outer Membrane Proteins / genetics
Bacterial Outer Membrane Proteins / immunology
Cross Reactions / immunology
Cytokines / blood
Endotoxins / metabolism
Meningitis, Meningococcal / metabolism
Meningitis, Meningococcal / prevention & control
Meningococcal Vaccines / genetics
Meningococcal Vaccines / immunology*
Mice
Neisseria meningitidis / classification
Neisseria meningitidis / genetics
Neisseria meningitidis / immunology*
Neisseria meningitidis, Serogroup B / genetics
Neisseria meningitidis, Serogroup B / immunology
Serotyping
Vaccines, Conjugate / genetics
Vaccines, Conjugate / immunology

Substances

Antigens, Bacterial
Bacterial Outer Membrane Proteins
Cytokines
Endotoxins
Meningococcal Vaccines
Vaccines, Conjugate

Grants and funding

This work was supported by the Military Infectious Disease Research Program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.